Introduction
Cuvettes are small, transparent containers used to hold liquid samples in spectrophotometry, fluorescence, and other optical measurements. Choosing the right cuvette is crucial for accurate results.
Types of Cuvettes & Their Uses
1. Material-Based Classification
- Glass Cuvettes – Affordable, best for visible range (340–2500 nm).
- Quartz Cuvettes – UV-transparent (190–2500 nm), ideal for DNA/RNA analysis.
- Plastic Cuvettes – Disposable, good for routine tests but less precise.
2. Specialized Cuvettes
- Fluorescence Cuvettes – Four clear sides for maximum light detection.
- Microvolume Cuvettes – Require only 1–2 µL of sample (used in NanoDrop).
- Flow-Through Cuvettes – For continuous monitoring in HPLC.
How to Select the Right Cuvette?
- Wavelength Range – Quartz for UV, glass/plastic for visible light.
- Path Length – Standard is 10mm, but shorter paths (1mm) reduce dilution needs.
- Sample Volume – Micro cuvettes for rare samples, standard for larger volumes.
Maintenance & Handling Tips
- Cleaning – Use ethanol for quartz, avoid acetone on plastic.
- Storage – Keep in a dust-free case to prevent scratches.
- Lifetime – Quartz lasts years, plastic is single-use.
Conclusion
Picking the right cuvette depends on wavelength, sample volume, and budget. Always match the cuvette to your instrument’s requirements.
