Introduction
A hemocytometer is a specialized slide used to count cells (RBCs, WBCs, platelets) under a microscope.
How Does a Hemocytometer Work?
- Grid System – Neubauer grid has 9 large squares for counting.
- Sample Loading – A coverslip creates a precise 0.1mm depth.
Step-by-Step Cell Counting
- Dilute the Sample – Usually 1:10 or 1:100 for blood.
- Load the Chamber – Use a pipette to fill both sides.
- Count Cells – Focus on the four corner squares (WBCs) or center square (RBCs).
- Calculate Concentration – Formula:Cells/µL=Count×Dilution Factor×104Cells/µL=Count×Dilution Factor×104
Common Mistakes & Fixes
- Overfilling – Causes uneven distribution.
- Bubbles – Leads to incorrect counts.
- Improper Cleaning – Residue affects future counts.
Automated vs. Manual Counting
- Pros of Manual Counting – Low cost, no machine needed.
- Pros of Automated – Faster, less human error.
Conclusion
Hemocytometers remain essential for accurate cell counting, especially in hematology and microbiology.
